To register for Mijn OV-chipkaart you need a personal or anonymous OV-chipkaart. It is not possible to register an account with a Businesscard.
Register
Register a username to get access to Mijn OV-chipkaart. Add your OV-chipkaart and apply for the online transaction overview, so you can use this the following day.
Activate account
Within a few minutes you will receive a confirmation email. Activate your account within 48 hours or your username will be removed.
Add card to account
Once your account is activated, you can add your OV-chipkaart and directly use the online services. If you have applied for the online transaction overview, you will be able to use it the following day.